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Immortalized Porcine Bone Marrow Mesenchymal Stromal Cell Line

Research & Design Tools
Cell Lines
College
College of Food, Agricultural, and Environmental Sciences (CFAES)
Researchers
Khatri, Mahesh
Licensing Manager
Dahlman, Jason "Jay"
614/292-7945
dahlman.3@osu.edu

TS-033666 — The cell line serves as a useful tool for examining mechanisms of tissue repair and supports the replication of newly identified porcine epidemics.

Porcine Epidemic Diarrhea Virus (PEDV) is a coronavirus that infects the cells lining the small intestine of a pig. PEDV has a substantial economic burden. It is highly infectious, and it is deadly to baby pigs from birth until about three weeks of age. Infected swine herds can suffer a 75-100% lo…

The Need

Porcine Epidemic Diarrhea Virus (PEDV) is a coronavirus that infects the cells lining the small intestine of a pig. PEDV has a substantial economic burden. It is highly infectious, and it is deadly to baby pigs from birth until about three weeks of age. Infected swine herds can suffer a 75-100% loss of baby pigs for a four to five week period. New vaccines could be developed utilizing an immortalized mesenchymal cell line to help protect against emerging viruses.

The Technology

A spontaneously immortalized bone marrow mesenchymal stromal cell line was generated by researchers at The Ohio State University, led by Dr. Mahesh Khatri, from the bone marrow of a four-week-old specific-pathogen free pig. The cells have been passaged numerous times in culture and express mesenchymal markers and lack the expression of hematopoetic markers. Extracellular vesicles derived from these cells will be examined as a cell-free therapy for human diseases in a pig model, and these cells will be useful as a substrate for the production of live-attenuated and killed viral vaccines. The cell line will serve as a useful tool for examining mechanisms of tissue repair, pharmacological and toxicological studies, and for the generation of mesenchymal stromal cell-derived extracellular vesicles.The cell line supports the replication of newly identified PEDV and deltacoronavirus.

Commercial Applications

  • Research tools
  • Vaccines
  • Agriculture

Benefits/ Advantages

  • Improved initial isolation of viral agents and killed and live attenuated vaccine production in cell culture
  • Support the replication of porcine epidemic diarrhea virus and swine deltacoronavirus
  • Useful as a substrate for the production of live-attenuated and killed viral vaccines