The Ohio State University Corporate Engagement Office

Back to All Technologies

Immortalized Swine Intestinal Epithelial Cell Line

Agriculture
Veterinary Medicine
Plant Varieties
College
College of Food, Agricultural, and Environmental Sciences (CFAES)
Researchers
Khatri, Mahesh
Saif, Linda
Saif, Yehia
Licensing Manager
Dahlman, Jason "Jay"
614/292-7945
dahlman.3@osu.edu

TS-037403 — An immortalized swine intestinal epithelial cell line, MK-LJS-OSU, derived from the duodenum of a newborn gnotobiotic pig.

Currently, most vaccines are produced in live embryonated eggs or primary cell cultures. Vaccine production using eggs has been done for more than half a century and can be slow, costly, and inefficient. Availability of a new cell line is likely to offer the industry a valuable alternative for pro…

The Need

Currently, most vaccines are produced in live embryonated eggs or primary cell cultures. Vaccine production using eggs has been done for more than half a century and can be slow, costly, and inefficient. Availability of a new cell line is likely to offer the industry a valuable alternative for producing vaccines.

The Technology

The Ohio State University researchers led by Dr. Mahesh Khatri have developed a spontaneously immortalized swine intestinal epithelial cell line from the duodenum of a newborn gnotobiotic pig. This cell line is well-established, replicates indefinitely, and can be held in a viable state in liquid nitrogen. The cell line would be suitable for replicating viruses and producing live and killed vaccines. These cells may be useful in a variety of research settings, such as in virus replication cycle studies, host-pathogen integration studies, cell-cell interaction studies, and bacterial adhesion studies.

Commercial Applications

  • Vaccine Production
  • Research

Benefits/ Advantages

  • Supports the replication of swine, human, and avian influenza virus
  • Cells grow rapidly in monolayers
  • Reduced time and space constraints compared to egg-based vaccine production and primary cell lines
  • Reduced risk of national vaccine shortages and contamination